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Description

Classic TEM. First description of the endoplasmic reticulum. Whole mount of unfixed, dried chick embryo fibroblast. Embryonic chick cells were grown on a thin collodion film supported by a fine mesh copper grid. After the cells had grown and spread out thin processes, the preparation was air dried. They reasoned that the thin processes would allow electrons to penetrate in the electron microscope. The only microscope available to them was owned by the Interchemical Corp. and housed in the Empire State Building. Fullam was the technician. The electron microscope was a first generation RCA type B. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi.

Biological Sources

NCBI Organism Classification
Gallus gallus
(red junglefowl)
Cell Type
fibroblast
CEF (chicken embryo fibroblast
Cellular Component
endoplasmic reticulum

Attribution

Names
Keith R. Porter
Albert Claude
Ernest F. Fullam
Published
J. Exp. Med. 81:233-246. 1945
Link
George E. Palade EM slide collection
Pubmed
19871454
Date
12/12/1952

Grouping

This image is part of a group.

Sample Preparation

Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 2000px ——
Y 1813px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.