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Description

Transmission electron micrograph showing the aggregation of rough endplasmic reticulum known as a Nissl bodiy within nerve cells of the superior cervical ganglion. Note that this very early (1953) micrograph is primarily of historical interest since it pre-dates glutaraldehye fixation which was introduced in the early 60's and is considered essential for adequate nerve tissue preservation. Examples of nerve tissue micrographs prepared in 1965 are CIL:37218, CIL:37219 and CIL:37220. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Additional reference: Palay, S and Palade, GE J Biophys Biochem Cytol. 1955 Jan;1(1):69-88. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 37,500x.

Biological Sources

Cell Type
neuron
Cellular Component
Nissl body
rough endoplasmic reticulum

Biological Context

Biological Process
cytoplasm organization
Human Development Anatomy
superior cervical ganglion

Attribution

Name
Sanford Palay
Link
George E. Palade EM Slide Collection
Date
00/05/1953

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data

Sample Preparation

Methods
chemically fixed tissue
Relation To Intact Cell
sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 6000px ——
Y 5526px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.