Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37223^* Cite

Description

Transmission electron micrograph showing the aggregation of rough endplasmic reticulum known as a Nissl bodiy within nerve cells of the superior cervical ganglion. Note that this very early (1953) micrograph is primarily of historical interest since it pre-dates glutaraldehye fixation which was introduced in the early 60's and is considered essential for adequate nerve tissue preservation. Examples of nerve tissue micrographs prepared in 1965 are CIL:37218, CIL:37219 and CIL:37220. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Additional reference: Palay, S and Palade, GE J Biophys Biochem Cytol. 1955 Jan;1(1):69-88. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 37,500x.

Biological Sources

Cell Type: neuron
Cellular Component: Nissl body; rough endoplasmic reticulum

Biological Context

Biological Process: cytoplasm organization
Human Development Anatomy: superior cervical ganglion

Attribution

Names: Sanford Palay
Link: George E. Palade EM Slide Collection

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL37223
Archival Resource Key (ARK): ark:/b7295/w9cil37223

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide
Processing History: unprocessed raw data

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	6000px	——
Y	5526px	——