The Cell: An Image Library - Image CIL:37221

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CIL:37221^*

Description

Transmission electron micrograph of the accumulation of rough endoplasmic reticulum known as Nissl bodies within nerve cells from the superior cervical ganglion. Note that this very early (1953) micrograph is primarily of historical interest since it pre-dates glutaraldehye fixation which was introduced in the early 60's and is considered essential for adequate nerve tissue preservation. Examples of nerve tissue micrographs prepared in 1965 are CIL:37218, CIL:37219 and CIL:37220. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Additional reference: Palay, S and Palade, GE J Biophys Biochem Cytol. 1955 Jan;1(1):69-88. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x7,600.

Biological Sources

Cell Type: neuron
Cellular Component: Nissl body; rough endoplasmic reticulum

Biological Context

Human Development Anatomy: superior cervical ganglion

Attribution

Link: George E. Palade EM Slide Collection
Date: 05/07/1953
Other: Sanford Palay

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide
Processing History: unprocessed raw data

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	6000px	——
Y	5358px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.