Licensing
Description
Transmission electron micrograph of mitochondria from guinea pig pancreas. The use of osmium tetroxide as a fixative for electron microscopy was first described by Dr. Palade at the Rockefeller. Another major advance in fixation for electron microscopy was done at Yale by David Sabatini who described the use of glutaraldehyde which not only preserved ultrastructure, but enzymatic activities which opened up the possibility of EM localization of enzymatic function in cells. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
Technical Details
References: Palade, G.E. 1952. J. Exp. Med. 95:285-298. Palade, G.E. 1952. Anat. Rec. 114:427-451. Sabatini, D.D, K. Bensch and R.J. Barrnett. 1963. J. Cell. Bio. 17:19-58. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 56,000.
Biological Sources
- NCBI Organism Classification
- Cavia porcellus
- (Guinea Pig)
- Cell Type
- pancreatic cell
- Cellular Component
- mitochondrion
- mitochondrial crista
- mitochondrial membrane
- mitochondrial matrix
Attribution
- Name
- George E. Palade
- Link
- George E. Palade EM Slide Collection
- Date
- 05/04/1968
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- transmission electron microscopy (TEM)
- Parameters Imaged
- electron density
- Source of Contrast
- differences in adsorption or binding of stain
Sample Preparation
- Methods
- osmium tetroxide fixed tissue
- Relation To Intact Cell
- sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 5998px | —— |
| Y | 4975px | —— |