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Description
This is Video S2 which corresponds to still images in top row of Figure 2D. It shows the spine dynamics of control neurons. Time-lapse confocal imaging was performed on DIV14 hippocampal neurons co-expressing GFP. Images were acquired every 1-minute using confocal microscopy. 3 frames/sec shown.
Technical Details
Confocal images were collected on an Olympus Fluoview 1000 microscope (IX81 base) equipped with a 60X/1.35 NA (oil)UPLSAPO 60X objective (Olympus). GFP was excited using the 488 nm laser line of a multi Ar laser. Fluorescence emission was collected using the following dichroic mirror/filter: SDM560/BA505–525 (GFP).
Biological Sources
- NCBI Organism Classification
- Rattus
- (Old World rats)
- Cell Type
- CNS neuron (sensu Vertebrata)
- Cellular Component
- dendritic spine
- dendrite
- dendritic shaft
- cytoplasm
Biological Context
- Biological Process
- dendritic spine development
- dendritic spine morphogenesis
- Molecular Function
- myosin II light chain binding
- ATPase activity
Attribution
- Names
- Jennifer L. Hodges
- Karen Newell-Litwa
- Hannelore Asmussen
- Miguel Vicente-Manzanares
- Alan Rick Horwitz
- Link
- http://www.plosone.org/article/info%3A...
- Date
- 00/26/2011
Grouping
This image is part of a group.Imaging
- Image Type
- time lapse microscopy
- Imaging Mode
- confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- EGFP
- Processing History
- unprocessed raw data
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 759px | .06μm |
| Y | 367px | .06μm |
| Time | 60 sec | 20 |
*CIL – Cell Image Library accession number. Please use this to reference an image.