Transmission electron micrograph of cell fractionation of guinea pig pancreas microsomes. Rough microsomes, the cell fractionation equivalent of the rough endoplasmic reticulum with bound ribosomes in intact cells, were extensively used to determine the mechanism of translocation of newly synthesized exportable proteins across the ER membrane. The culmination of these studies on rough microsomes was the elucidation of the "Signal Hypothesis". Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Relevant citations: Siekevitz, P. and G. E. Palade.1958. J. Biochem. Biophys. Cytol. 4:309-318. Palade, G.E., and P. Siekevitz. 1956. J. Biochem. Biophys. Cytol. 2:671-690. Palade. G.E. 1955. J. Biochem. Biophys. Cytol. 1:59-68. Redman, C.M., P. Siekevitz, and G.E. Palade. 1966. J. Biol. Chem. 241:1150-1158. Sabatini, D.D. and G. Blobel. 1970. J. Cell. Biol. 45:146-157.
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