Electron microscope autoradiograph taken 20 min after injection showing that the radiographic label has migrated from the rough endoplasmic reticulum to the Golgi complex. Most of the radioactive grains are over condensing vacuoles and the perpheral clusters of small vesicles have much less activity. Image corresponds to Figure 5 in J. Cell Biol. 20:473-495. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
Anesthetized animals were intravenously injected with 1-5 millicuris of DL-leucine 4,5-H^3 with a specific activity of 3570 mc/mM. At times ranging from 4 min to 15 hrs after injection the pancreas was removed and fixed in osmium tetroxide. Tissue was embedded in methacrylate, sectioned, and post-stained with uranyl acetate. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x30,000.