Licensing
Description
Image is of a section of a liver from a CD-1 mouse treated intravenously with siRNA-Cy5 enapsulated with lipid nanoparticle (LNP201-siRNA-Cy5) for 2 hours. siRNA-Cy5 (red) can be observed in the sinusoids and inside hepatocytes. Cells are outlined with phalloidin-Alexa488 (green) and nuclei are stained with DAPI (blue). The black section in the lower right corner is a vein of the liver. This image is Fig 2F of J Histochem Cytochem 2011 59: 727 and Cover Image for the Journal of Histochemistry and Cytochemistry, August 2011.
Technical Details
7 micron thick cryosection of liver was fixed with 4% paraformaldehyde in PBS for 10 min. Sections were stained with phalloidin-Alexa 488 and DAPI. Images were captured with an Olympus BX61 microscope with a 1.4 mega-pixel progressive scan camera.
Biological Sources
- NCBI Organism Classification
- Mus musculus
- (house mouse)
- Cell Type
- hepatocyte
- sinusoids
- Cellular Component
- filamentous actin
- nucleus
Attribution
- Names
- Bin Shi
- Ed Keough
- Andrea Matter
- Karen Leander
- Stephanie Young
- Ed Carlini
- Alan B. Sachs
- Weikang Tao
- Marc Abrams
- Bonnie Howell
- Laura Sepp-Lorenzino
- Published
- J Histochem Cytochem 2011 59: 727
- Link
- http://jhc.sagepub.com/content/59/8/72...
- Pubmed
- 21804077
Imaging
- Image Type
- recorded image
- Imaging Mode
- widefield illumination
- fluorescence microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- compartmentalization of stain or label
- Visualization Methods
- Cy5
- Alexa Fluor 488
- 4',6-diamidino-2-phenylindole (DAPI)
- Data Qualifiers
- processed data
Sample Preparation
- Methods
- formaldehyde fixed tissue
- Relation To Intact Cell
- cryostat-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 1344px | —— |
| Y | 1024px | —— |