Image is of a section of a liver from a CD-1 mouse treated intravenously with siRNA-Cy5 enapsulated with lipid nanoparticle (LNP201-siRNA-Cy5) for 2 hours. siRNA-Cy5 (red) can be observed in the sinusoids and inside hepatocytes. Cells are outlined with phalloidin-Alexa488 (green) and nuclei are stained with DAPI (blue). The black section in the lower right corner is a vein of the liver. This image is Fig 2F of J Histochem Cytochem 2011 59: 727 and Cover Image for the Journal of Histochemistry and Cytochemistry, August 2011.
7 micron thick cryosection of liver was fixed with 4% paraformaldehyde in PBS for 10 min. Sections were stained with phalloidin-Alexa 488 and DAPI. Images were captured with an Olympus BX61 microscope with a 1.4 mega-pixel progressive scan camera.