Alternate header for print version


Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36786*  Cite 
Description

Details of the origins of the cytopharyngeal ribbons and the quadrulus associated ribbons. At first view the cytopharyngeal ribbons look like they might arise next to basal bodies but it is clear that the cytopharyngeal ribbons arise only from the filamentous reticulum. The quadrulus associated ribbons appear to be analogous to the postciliary microtubules of the somatic ciliature that they curve posteriorly from the quadrulus. However quadrulus associated ribbons do pass very close or even touch the ends of the cytopharyngeal ribbons without becoming part of these latter ribbons. Discoidal vesicles bind to the anterior sides (observer’s left) of the cytopharyngeal ribbons and the smaller set of ribbons lies at its posterior side against the cytopharyngeal membrane. The tubular fingers of the alveoli pass between the cytopharyngeal ribbons next to the nodes of the filamentous reticulum from which the cytopharyngeal ribbons originate. Finally a row of parasomal sacs is seen along the dorsal edge of the quadrulus from which coated vesicles form. TEM taken on 5/23/73 by R. Wolf with Hitachi HU11A operating at 75kV. Neg. 16,250X. Bar = 0.5µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39176) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
oral apparatus
Biological Context
Biological Process
oral apparatus organization
Attribution
Names
Richard Allen (University of Hawaii)
R. Wolf
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36786
Archival Resource Key (ARK)
ark:/b7295/w9cil36786
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2320px ——
Y 2603px ——