Following digestion, that only occurs in the phagolysosome (DV-III), the membrane of these DVs undergoes extensive tubulation. These tubules are ~45nm in diameter and arise from the cytosolic surface of the DV-III in clumps of 3 to 6 tubules per clump. The tubules contain a glycocalyx-like lining in their lumens which leaves only a 10nm diameter opening in the center of the tubule (see Allen and Fok, J. Cell Biol. 99:1955-1959, 1984). TEM taken on 4/13/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,750X. Bar = 0.25µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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