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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36751*  Cite 

Phagoacidosomes are heavily labeled for L1 antigen in 5-8 to 15-18 minute-old DVs. However, only some DVs at 15-18 min still had L1 label. Only occasionally did we see tubules with label arising from the 15-18 min-old vacuole to support the idea that DV-II membrane is retrieved during the transformation of phagoacidosomes to phagolysosomes. The DV shown here was 15-18 min old as it contained 0.3µm latex beads. There is a small amount of DV-II specific L1 immunogold label in the tubules and in their bulbous ends. The phagolysosome membrane itself is totally free of this label. TEM taken on 2/18/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm.

Technical Details

Glutaraldehyde fixation followed freezing and microtomy. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
Biological Context
Biological Process
digestive system process
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2154px ——
Y 2478px ——