Phagoacidosomes are heavily labeled for L1 antigen in 5-8 to 15-18 minute-old DVs. However, only some DVs at 15-18 min still had L1 label. Only occasionally did we see tubules with label arising from the 15-18 min-old vacuole to support the idea that DV-II membrane is retrieved during the transformation of phagoacidosomes to phagolysosomes. The DV shown here was 15-18 min old as it contained 0.3µm latex beads. There is a small amount of DV-II specific L1 immunogold label in the tubules and in their bulbous ends. The phagolysosome membrane itself is totally free of this label. TEM taken on 2/18/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm.
Glutaraldehyde fixation followed freezing and microtomy. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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