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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36749*  Cite 

A DV-III contains a thick continuous glycocalyx (polysaccharide coat) on the luminal side of its membrane as well as paracrystalline material, both provided by the fused lysosomes. The latex beads indicate that this vacuole was 27-30 min old. The cell had been exposed to HRP for the first 3 minutes but no HRP reaction product remained in the DV. TEM taken on 4/8/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,750X. Bar = 0.25µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
food vacuole
Biological Context
Biological Process
digestive system process
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2220px ——
Y 2586px ——