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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36734*  Cite 

Quick-freeze deep-etch of acidosomes show the results of carrier vesicle fusion with the acidosomes. Both the E-face and P-face of the acidosomes appear to have two areas, the larger area on the E-face has few IMPs but has pits left by the removal of IMPs during fracturing and another area that is smooth with fewer pits that resembles a fused carrier vesicle membrane. A line marking the fusion site between these two vesicles is evident. The P-face of the acidosome has many IMPs while the fused carrier vesicle membrane is smooth. Presumably these two membrane areas will mix with time. Thus acidosomes, which are somewhat similar in appearance to multivesicular bodies in higher organisms, are late endosomes and deliver their membrane, their contents and their V-ATPase complexes to the phagosome to transform the phagosome to the phagoacidosome. TEM taken on 7/29/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 24,800X. Bar = 0.2µm. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.

Technical Details

A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40563) is available for quantitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
early phagosome
vesicle membrane
Biological Context
Biological Process
vesicle docking
digestive system process
Richard Allen (University of Hawaii)
C. Schroeder
J Cell Sci. 1993 Sep;106 ( Pt 1):411-22.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
shadowing and plating
recorded image
Print of negative scanned for Photoshop
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2136px ——
Y 2700px ——