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CIL:36728*  Cite 

To see if acidosomes contain hydrolases (digestive enzymes) we incubated cells in Gomori’s medium which in the presence of acid phosphatase and lead will form an electron-opaque reaction product. Acidosomes surrounding a DV-I are free of reaction product even though a nearby DV, presumably a DV-III, contains abundant reaction product. A few vesicles assumed to be lysosomes also contain some reaction product. This experiment demonstrated that acidosomes are not lysosomes and do not contain digestive enzymes at least not AcPase. TEM taken on 4/8/83 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,000X. Bar = 1µm.

Technical Details

Glutaraldehyde fixation followed by Gomori acid phosphatase treatment and dehydration in alcohol and embedding in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
early phagosome
transport vesicle
Biological Context
Biological Process
digestive system process
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2136px ——
Y 2652px ——