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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36726*  Cite 

At the same time that the acidosomes fuse with the phagosome, the DV-I membrane, that originated from the discoidal vesicles, is transformed into tubules that are removed from the phagosome. By this process the DV-I is transformed from a phagosome into a phagoacidosome (DV-II), a process of apparently “total” membrane replacement. (For discussion see Allen et al., J. Cell Sci. 108:1263-1274, 1995). The mAb to antigen Q2 specifically labels the tubules that are being removed from the DV-I. TEM taken on 9/18/93 by R. Allen with Zeiss 10A TEM. Neg. 12,000X. Bar = 0.5µm. Part published in J. Cell Sci. 108:1263-1274, 1995. Adapted with permission.

Technical Details

To label membranes inside the cell we used very lightly fixed cells (0.25% glutaraldehyde) that were then rapidly frozen in liquid nitrogen and sectioned later at -100oC. These frozen sections were picked up on drops of methylcellulose and transferred to a Formvar-supported grid. The sections were immunogold labeled (15nm gold) to show the location of the specific antigen inside the cell as well as on the cell surface. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40558) which is available for quantitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
food vacuole
phagocytic vesicle
Biological Context
Biological Process
digestive system process
Richard Allen (University of Hawaii)
J Cell Sci. 1995 Mar;108 ( Pt 3):1263-74.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2160px ——
Y 2454px ——