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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36714*  Cite 
Description

Two of the three types of vesicles that are transported to the cytopharynx and that accumulate in large numbers at the cytopharynx are shown in this electron micrograph. The discoidal vesicles move up to the edge of the cytopharynx membrane where the cytopharynx membrane is continuous with the plasma membrane of the buccal cavity. Here the discoidal vesicles fuse with the cytopharynx membrane. Just behind this edge is found a layer of larger vesicles, the acidosomes, which dock with the cytopharynx membrane as soon as they make contact with this membrane. Unlike the discoidal vesicles these acidosomes do not fuse with the cytopharynx or nascent vacuole until the phagosome has pinched off and moved toward the posterior pole of the cell. TEM taken on 4/8/83 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 8,000X. Bar = 0.5µm.

Technical Details

After a light glutaraldehyde fixation, these organisms were incubated in Gomori reagent to reveal the presence of acid phosphatase then dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cytostome
transport vesicle
food vacuole
Biological Context
Biological Process
digestive system process
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36714
Archival Resource Key (ARK)
ark:/b7295/w9cil36714
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2298px ——
Y 2496px ——