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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36702*  Cite 
Description

Structures at the left edge of the cytopharynx arise in contact with the filamentous reticulum that lies next to the alveoli to the side of the quadrulus. The cytopharyngeal ribbons coming out of the filamentous reticulum have a spacing that seems to be determined by the lattice-like arrangement of this reticulum. A microtubular ribbon arises against each electron-opaque row of nodes of the reticulum. A cytostomal cord borders the cytosolic ends of these ribbons along the entire length of the left side of the cytopharynx. The ribbons curve sharply over the cord and pass out into the endoplasm. Three types of vesicles are transported along these ribbons, discoidal vesicles, large acidosomes and small 100nm carrier vesicles. They all move along the ribbons from the endoplasm toward the cytopharynx and the cytostomal cord toward the origins of the microtubules. Discoidal vesicles reach the cytopharyngeal membrane under the cord where they fuse. Acidosomes are too large to pass under the cord and the 100nm carrier vesicles appear to have an affinity for the acidosomes and fuse with these large vesicles before reaching the cytopharyngeal membrane. TEM taken on 3/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 8,500X. Bar = 0.5µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
oral apparatus
cell cortex part
microtubule associated complex
Biological Context
Biological Process
oral apparatus organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36702
Archival Resource Key (ARK)
ark:/b7295/w9cil36702
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2976px ——
Y 3360px ——