Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36699^* Cite

Description

A view of the right side of the cytopharynx showing the groove and its associated microtubules running longitudinally along the groove (for cross sections see Allen, J. Ultrastruct. Res. 63:64-78, 1980, Fig. 4) and bundles of postoral microtubules on its cytosolic side. This cell was fed latex beads and treated with DMSO and then incubated in DAB so that discoidal vesicles appear electron opaque. Some discoidal vesicles can be found along the longitudinally oriented microtubules of the groove. The nascent food vacuole has many acidosomes docked at its surface even at the right side of the cytopharynx. TEM taken on 9/14/82 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 8,500X. Bar = 0.5µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: oral apparatus; food vacuole; microtubule associated complex

Biological Context

Biological Process: oral apparatus organization; microtubule-based process; response to food; microtubule-based transport

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36699
Archival Resource Key (ARK): ark:/b7295/w9cil36699

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3096px	——
Y	3464px	——