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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36695*  Cite 
Description

Confocal z-stack of a dividing zebrafish embryo. The fixed embryo is visualized using EMTB-3GFP (microtubules-marker - green), phalloidin (actin marker -red) and Hoechst (DNA marker - blue).

Technical Details

Images collected on a Zeiss LSM 710 confocal with a 20X 0.8 NA objective using 561, 633, and 750 nm laser lines.

Biological Sources
NCBI Organism Classification
Danio rerio
Cell Type
embryo
Cellular Component
microtubule
DNA
actin cytoskeleton
Biological Context
Biological Process
mitosis
Attribution
Names
Martin Wuehr
Timothy Mitchison
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36695
Archival Resource Key (ARK)
ark:/b7295/w9cil36695
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
distribution of a specific protein
Visualization Methods
labeled primary antibody
phalloidin
hoechst
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2048px 0.3459µm
Y 2048px 0.3459µm