The Cell: An Image Library - Image CIL:36670

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CIL:36670^*

Description

In a conventional freeze fracture view the membrane around the trichocyst tip is decorated with striations and IMPs. These decorations are found at the site where the trichocyst tip is associated with the alveolar membrane in the septum. Pores in the septum connect alveoli and organized IMPs at the base of the cilium indicate an association of the ciliary membrane with the proximal ends of the 9 doublets of the cilium and with the transition zone between the basal body and cilium. TEM taken on 9/27/78 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,750X. Bar = 0.25µm.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: plasma membrane; trichocyst

Biological Context

Biological Process: plasma membrane organization

Attribution

Name: Richard Allen (University of Hawaii)
Link: http://www5.pbrc.hawaii.edu/allen/

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop

Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Relation To Intact Cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2196px	——
Y	2526px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.