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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36669*  Cite 
Description

A favorable view of one trichocyst docking site in a quick-freeze deep-etch image shows the prominent outer ring of 37 IMPs and about the same number of IMPs in the inner ring more deeply embedded in the cytosolic leaflet. Pits, where the rosette was removed during fracturing of the chemically-unfixed rapid-frozen cell, are seen at the center of the docking site. Two gaps in the rings indicate the site of the septum in the underlying alveoli where the plasma membrane is not linked to the alveolar rims by the ring IMPs. TEM taken on 2/15/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm.

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
plasma membrane
trichocyst
Biological Context
Biological Process
plasma membrane organization
trichocyst docking site
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36669
Archival Resource Key (ARK)
ark:/b7295/w9cil36669
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
elevation
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
freeze_fracture/freeze_etch
shadowing and plating
recorded image
film
Print of negative scanned for Photoshop
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2154px ——
Y 2742px ——