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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36666*  Cite 

A relatively low magnification view of the anterior end of Paramecium showing the placement and size of trichocysts relative to the cortex and cytoplasmic organelles. Trichocysts docked at the crests of the surface ridges have “carrot” shaped bodies surmounted by a spike that is long enough to penetrate from below the infraciliary lattice all the way to the tip of the ridge. Two trichocysts have been internally discharged into elongated shafts during fixation. cv, contractile vacuole. TEM taken on 12/2/83 by R. Allen with Zeiss 10A operating at 80kV. Neg. 1,600X. Bar = 2µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
Biological Context
Biological Process
organelle organization
trichocyst distribution
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2322px ——
Y 2658px ——