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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36663*  Cite 
Description

In one experiment in which electric shock was applied to Paramecium cells to trigger trichocyst discharge the macronucleus in one cell was observed to undergo morphological changes of two types. First, a large area of the nucleus, normally found to contain electron-opaque foci, had changed into whorls of more electron-transparent filamentous material. Secondly, a section of the nuclear envelope had folded into a stack like the annulate lamellae seen in oocytes of many vertebrate and invertebrate cells (see Kessel, Int. Rev. Cytol. 82:181-303, 1983). The nuclear pores were present in part of this stack of the nuclear envelope while in other parts the pores were reduced or absent and a layer of dots or lines occupied the nucleoplasmic space between the two envelope segments. Presumably these annulate lamellae represent a temporary storage mechanism for excess nuclear envelope. TEM taken on 2/28/84 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
macronucleus
nuclear pore
Biological Context
Biological Process
macronucleus organization
nuclear pore distribution
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36663
Archival Resource Key (ARK)
ark:/b7295/w9cil36663
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2708px ——
Y 3000px ——