Grazing thin section through the nuclear envelope of a macronucleus. Nuclear pores are packed so tightly that they often form a hexagonal array with 6 pores surrounding a central pore. The pores often have electron opaque dots in their centers which indicate passage of ribonucleoprotein particles (RNP) between the nucleus and the cytoplasm. TEM taken on 1/4/82 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 17,500X. Bar = 0.2µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).