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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36645*  Cite 
Description

Like other ciliates P. multimicronucleatum has two morphologically and functionally different nuclei. Shown here is the tiny micronucleus, of which there are four, during interphase of this cell. This nucleus has the task of dividing the nuclear genome between the two daughter cells during mitosis and in forming the two haploid pronuclei one of which will be exchanged with a partner cell during conjugation. However, during interphase this nucleus is not involved in forming ribosomes or messenger RNA so it does not control the metabolic processes of the cell. This micronucleus, much smaller than in other paramecia such as P. caudatum, has a central condensed mass of chromatin surrounded by a less dense nucleoplasm. This is known as a vesicular type of micronucleus. There is a typical nuclear envelope with numerous pores. This micronucleus was from a cell in day 5 of a culture age study where cells are in mid-log phase of growth. The cell is presumably undergoing rapid metabolism. TEM taken on 5/29/78 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X. Bar = 0.5µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
micronucleus
cytoplasm
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36645
Archival Resource Key (ARK)
ark:/b7295/w9cil36645
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2819px ——
Y 3000px ——