Large patches of rough ER surrounded by Golgi stacks (visible only at high magnification) have formed in the cytosol along with fields of lipid bodies. By day 2 these ER and lipid fields will be dispersed or depleted. For culture age study see Fok and Allen, Eur. J. Cell Biol. 25:182-193, 1981. TEM taken on 4/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 4,050X. Bar = 1µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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