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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36637*  Cite 
Description

Like other cells Paramecium has peroxisomes. These organelles are usually smaller than mitochondria and have a granular appearance and contain a few tubular-like inclusions. These tubules have not been conclusively shown to be continuous with the single limiting membrane of this organelle. In this case the peroxisome has a microtubule lying along its surface. This cell was exposed to HRP for 30 seconds and then chased in HRP-free medium for 3 minutes. The subsequent incubating with hydrogen peroxidase and diaminobenzidine resulted in an electron opaque reaction product in the peroxisomes not because they took in HRP but because the peroxisomes contain large amounts of catalase that results in the reduction of hydrogen peroxidase. HRP can only enter the living cell by endocytosis, phagocytosis, when trichocysts are discharged, at the open cytoproct or when a CV opens. TEM taken on 6/13/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 22,500X. Bar = 0.25µm.

Technical Details

This cell was exposed to HRP for 30 seconds and then chased in HRP-free medium for 3 minutes. The subsequent incubating with hydrogen peroxidase and diaminobenzidine resulted in an electron opaque reaction product in the peroxisomes not because they took in HRP but because the peroxisomes contain large amounts of catalase that results in the reduction of hydrogen peroxidase. This was followed by standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
peroxisome
cytoplasm
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36637
Archival Resource Key (ARK)
ark:/b7295/w9cil36637
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2664px ——
Y 3000px ——