Protozoa, including P. multimicronucleatum, unlike most cells, have tubular cristae in their mitochondria. Tubular cristae, however, do occur occasionally in higher organisms such as in the lutein cells and testes interstitial cells of humans. Mitochondria are particularly numerous in the cortical region of paramecia. Collidine-buffered fixative. This image includes trichocysts that have expanded internally during fixation. TEM taken on 12/18/68 by R. Allen with Philips 300 operating at 80kV. Neg. 16,000X. Bar = 0.5µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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