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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36618*  Cite 
Description

Immunogold labeled thin frozen section of P. multimicronucleatum. Gold lies over the microtubules of the basal bodies and the doublets of the cilia. Also short segments of the transverse and postciliary microtubules are labeled. TEM taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm.

Technical Details

Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39322) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
microtubule basal body
microtubule associated complex
Biological Context
Biological Process
cortical cytoskeleton organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36618
Archival Resource Key (ARK)
ark:/b7295/w9cil36618
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
gold
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2262px ——
Y 2130px ——