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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36430*  Cite 
Description

Intracellular dye injections of protoplasmic a 1-week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Lucifer Yellow (green) and immunostained to reveal the localization of glial-specific proteins S-100 (red) and GFAP (blue). Optical sections were generated with a single photon confocal microscope. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Intracellular dye injections of protoplasmic a 1-week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Lucifer Yellow (green) and immunostained to reveal the localization of glial-specific proteins S-100 (red) and GFAP (blue). Optical sections were generated with a single photon confocal microscope.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
astrocyte of the hippocampus
astrocyte
Cellular Component
cell projection cytoplasm
cell body
Biological Context
Biological Process
forebrain astrocyte development
cell projection organization
Attribution
Names
Eric A. Bushong
Maryann E. Martone
Mark H. Ellisman
Pubmed
PMID:15036382
Link
CCDB 1072
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36430
Archival Resource Key (ARK)
ark:/b7295/w9cil36430
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
intracellular filling
Visualization Methods
lucifer yellow
Alexa Fluor 568
X-Rhodamine
primary antibody plus labeled secondary antibody
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1052px ——
Y 1052px ——