Licensing

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Description

Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 4 week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Alexa 488 (green) and Alexa 568 (red). Optical sections were generated with a single photon confocal microscope and compiled to produce this maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 4 week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Alexa 488 (green) and Alexa 568 (red). Optical sections were generated with a single photon confocal microscope and compiled to produce this maximum projection image.

Biological Sources

NCBI Organism Classification
Rattus norvegicus
(Brown Rat)
Cell Type
astrocyte
Cellular Component
cell projection cytoplasm
cell body

Biological Context

Biological Process
forebrain astrocyte development
cell projection organization

Attribution

Names
Eric A. Bushong
Maryann E. Martone
Mark H. Ellisman
Link
CCDB 1053
Pubmed
15036382

Grouping

This image is representative of a group of similar images.

Imaging

Image Type
recorded image
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
intracellular filling
Visualization Methods
Alexa Fluor 488
Alexa Fluor 568
Data Qualifiers
processed data

Sample Preparation

Methods
formaldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 1052px ——
Y 1052px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.