Immunofluorescent pictures of Tetrahymena labeled with an antibody to the V-ATPase of Dictyostelium discoideum provided by Dr. T. L. Steck, University of Chicago. a. This polyclonal antibody was the same as that used in Fok et al., J. Cell Sci. 108:3163-3170, 1995, to label the decorated tubules of Paramecium. The decorated tubules form a striated cap over the posterior end of the cell but leave the CV pore and the CV itself unlabeled. b. During division the cap is reduced in size in the opisthe while in figure c a new field of decorated tubules appears near the equatorial region of the dividing cell near the posterior end of the proter. d. Both decorated-tubule regions grow in size and in intensity as division proceeds. Fluorescent micrographs taken by M. Aihara on 12/6/91 and 1/3/92. Unpublished results. Bars = 20µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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