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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36284*  Cite 
Description

At its most distal extremity the stalk ends in a clump of sheath-like fibers with embedded bacteria. The spasmoneme itself seems to end in a tight association with its surrounding plasma membrane. This is possibly the only point where the myonemal fibers meet the plasma membrane without an intervening alveolus. However there may be microtubules or modified basal bodies linking the spasmonemal fibers to the plasma membrane. The outer surface of the plasma membrane shows striations (at high magnification) next to the holdfast. TEM taken on 2/16/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.5µm. Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39512) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Vorticella convallaria
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component
myofibril
spasmoneme
Biological Context
Biological Process
muscle system process
Attribution
Names
Richard Allen (University of Hawaii)
Published
J Cell Biol. 1973 Feb;56(2):559-79.
Pubmed
4630196
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36284
Archival Resource Key (ARK)
ark:/b7295/w9cil36284
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 3048px ——
Y 3294px ——