A cross-section and tangential views of three other linkage complexes. The mid piece sits in an indentation of the ER membrane on a bed of 5 slats that pass through the membrane. Rails affix the ER membrane to the myoneme and prevent the vesiculated ER from moving away from the myoneme. Beautifully preserved views of cross sections of cilia. TEM taken on 3/7/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 28,500X. Bar = 0.2µm.Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39502) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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