V. convallaria preserved in collidine buffered fixative (not cacodylate) has a less dense cytoplasm in which oranelles are easier to recognize. The rough ER has vesiculated but this vesiculation enhances the identification of loci where ER remains bound to the myonemes. These loci on the ER are called “linkage complexes." These complexes are unique and have not been reported outside the peritrichs. Macronucleus with nucleolus and cytopharynx at its distal end. TEM taken on 3/30/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,750X. Bar = 0.5µm. Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39461) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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