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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36269*  Cite 
Description

Cytopharynx with numerous folded membrane ribs subtended by five or so microtubules. These microtubules have arms that bind to other microtubules and to the membrane of the cytopharynx and to surrounding vesicles of various sizes. The vesicles are uncoated and free of ribosomes. The vesicles are probably equivalent to the discoidal vesicles and/or to the 100nm vesicles; the latter arise from the endosomal pathway of Paramecium. Evidence for vesicle fusion to the cytopharynx membrane, between the ribs, can be seen. Bacteria and tips of cilia are present in the lumen of the cytopharynx. TEM taken on 4/6/72 by R. Allen with Hitachi HU11A operating at 75kV. Mag. 19,500X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39460) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Vorticella convallaria
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component
oral apparatus
microtubule associated complex
plasma membrane
Biological Context
Biological Process
oral apparatus organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36269
Archival Resource Key (ARK)
ark:/b7295/w9cil36269
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 3168px ——
Y 3286px ——