The acidosome-like vesicle has a bull’s eye appearance with the invagination appearing in the middle of the vesicle. Other vesicles contain multiple layers resembling multilipid layers. Two vesicles with thick membranes and flocculent material may be lysosomes. Membranes of lysosomes of other cells tend to be thicker and have a polysaccharide luminal coat and do not have invaginations. TEM taken on 7/15/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39275) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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