The Cell: An Image Library - Image CIL:36072

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CIL:36072^*

Description

Figures 440 (upper) and 441 (lower) from Chapter 16 (Cytoplasmic matrix and cytoskeleton) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Examples of the dense cytoskeletal network in the periphery of cells in vitro. In the upper panel, two microtubules traverse the meshwork of actin filaments. This cell was prepared by treated with Triton to extract soluble proteinaceous material, washed, quick frozen with liquid helium, deep etched, and rotary shadowed. Image by John Heuser. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.

Biological Sources

NCBI Organism Classification: Mus musculus; (house mouse)
Cell Type: fibroblast
Cell Line: 3T3-Swiss albino
Cellular Component: actin cytoskeleton; cytoplasmic microtubule

Biological Context

Biological Process: actin cytoskeleton organization; microtubule cytoskeleton organization

Attribution

Names: Don W. Fawcett; John Heuser
Published: ISBN 0721635849
Link: http://www.ascb.org/bioeducate/Fawcett...

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM)
Imaging Mode: illumination by electrons; detection of electrons
Parameters Imaged: electron density
Source of Contrast: differences in deposition of metal shadow

Data Qualifiers: processed data

Sample Preparation

Methods: detergent permeabilized; FBbi:00000621
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	891px	——
Y	1257px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.