Licensing

Attribution Non-Commercial; No Derivatives:This image is licensed under a Creative Commons Attribution, Non-Commercial, No Derivatives License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36067^* Cite

Description

A thinly-spread human skin fibroblast stained by indirect immunofluorescence with actin-specfic antibody. Actin bundles correspond with stress fibers, extending out into the periphery of the cell. Figure 435 from Chapter 16 (Cytoplasmic matrix and cytoskeleton) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Image from Elias Lazarides, J. Cell Biol. 65:549-61, 1975, PMID: 1094020. A PDF copy of the accompanying chapter is available on the ASCB’s BioEDUCATE website.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: fibroblast
Cellular Component: actin filament bundle; stress fiber

Biological Context

Biological Process: actin cytoskeleton organization

Attribution

Names: Don W. Fawcett; Elias Lazarides
Published: ISBN 0721635849
Link: http://www.ascb.org/bioeducate/FawcettTheCell...

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36067
Archival Resource Key (ARK): ark:/b7295/w9cil36067

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: primary antibody plus labeled secondary antibody
Data Qualifiers: processed data

Sample Preparation

Methods: crosslinking-fixative fixed tissue; detergent permeabilized
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	888px	——
Y	1240px	——