The Cell: An Image Library - Image CIL:35968

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CIL:35968^*

Description

Figures 335 (upper) and 336 (lower) from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Upper, cross-section of guinea pig sperm tail at the level of the midpiece; lower, cross-section at the level of the principal piece. Both samples were prepared using aldehyde fixation in the presence of tannic acid. When followed with osmium and uranyl acetate staining, this procedure improves contrast but may result in thickening of the doublets, arms and spokes. Upper image by Daniel Friend; lower from David Phillips. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.

Biological Sources

NCBI Organism Classification: Cavia porcellus; (Guinea Pig)
Cell Type: sperm
Cellular Component: axoneme; flagellum; axonemal microtubule

Biological Context

Biological Process: fertilization

Attribution

Names: Don W. Fawcett; Daniel Friend; David Phillips
Published: ISBN 0721635849
Link: http://www.ascb.org/bioeducate/Fawcett...

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM)
Imaging Mode: illumination by electrons; detection of electrons
Parameters Imaged: electron density
Visualization Methods: osmium tetroxide; uranyl salt

Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	876px	——
Y	1278px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.