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Description
A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged TEM1. TEM1 is a GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis. These phase, gfp images are part of an image group that ranges from CIL:35900-35919. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
Technical Details
Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
Biological Sources
- NCBI Organism Classification
- Saccharomyces cerevisiae
- (Baker's Yeast, Brewer's Yeast)
- Cellular Component
- TEM1 protein
- spindle pole body
Biological Context
- Biological Process
- regulation of exit from mitosis
- cell cycle
- cell division
- mitosis
- small GTPase mediated signal transduction
- Molecular Function
- GTP binding
- nucleotide binding
- GTPase activity
- protein binding
Attribution
- Names
- David Ball
- Jean Peccoud
- Published
- PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
- Pubmed
- 22046265
Grouping
This image is representative of a group of similar images.Imaging
- Image Type
- recorded image
- Imaging Mode
- fluorescence microscopy
- phase contrast microscopy
- Parameters Imaged
- fluorescence emission
- elastic scattering of electrons
- Source of Contrast
- distribution of a specific protein
- differences in amount of elastic light scattering
- Visualization Methods
- EGFP
- visualization of contiguous regions
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 512px | 213.4nm |
| Y | 512px | 213.4nm |
| Time | 300 sec | 61 |
*CIL – Cell Image Library accession number. Please use this to reference an image.