Licensing

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Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc15, a protein kinase involves in cytokinesis. These phase and GFPimages are part of an image group containing CIL 35840-35859. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.

Technical Details

The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please read the microscopy section of the referenced paper for details before image analysis.

Biological Sources

NCBI Organism Classification
Saccharomyces cerevisiae
(Baker's Yeast, Brewer's Yeast)
Cellular Component
CDC15 Protein
cellular bud neck
spindle pole body

Attribution

Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265

Grouping

This image is representative of a group of similar images.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Time 300 sec 61
*CIL – Cell Image Library accession number. Please use this to reference an image.