The Cell: An Image Library - Image CIL:35794

Licensing

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CIL:35794^*

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Bck2, a Cln-independent activator of CLN1,2 expression. These phase and GFP images are part of a group that ranges from CIL:35780-35799. Note that there are additional groups of images monitoring the dynamics of other proteins involved in cell cycle regulation by the same authors in the Library.

Technical Details

The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Read the microscopy section of the referenced paper for details prior to image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae; (Baker's Yeast, Brewer's Yeast)
Cellular Component: BCK2 protein

Biological Context

Biological Process: regulation of cell cycle; establishment of protein localization

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26
Pubmed: 22046265

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP; visualization of contiguous regions
Processing History: unprocessed raw data

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4nm
Time	300 sec	61

*CIL – Cell Image Library accession number. Please use this to reference an image.