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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35739*  Cite 

This is a time lapse image series of Saccharomyces cerevisiae expressing GFP tagged Bub2, a GTPase activator involves in mitotic cell cycle spindle orientation checkpoint and regulation of exit from mitosis. These phase and GFP images are part of an image group containing CIL 35720-35739. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.

Technical Details

Images were acquired using a 60x 1.42 NA objective lens on a Delta Vision system equipped with a CoolSNAP_HQ2 / HQ2-ICX285. Please read the microscopy section of the referenced paper for details before image analysis.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cellular Component
BUB2 protein
Biological Context
Biological Process
mitotic spindle orientation checkpoint
establishment of protein localization
Molecular Function
GTPase activator activity
David Ball
Jean Peccoud
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
phase contrast microscopy
Parameters Imaged
fluorescence emission
elastic scattering of photons
Source of Contrast
distribution of a specific protein
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Time 300 seconds 61