A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged NET1. NET1 is a core subunit of the RENT complex that involves in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure. These phase, gfp images are part of an image group that ranges from CIL:35700-35719. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.