Alternate header for print version


Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35618*  Cite 
Description

The relationship between the actin cytoskeleton (blue), adherens junctions (green) and phospho-Myosin Light Chain (MLC) (red) after 30 minutes of calcium stimulation.

Technical Details

The specimen was fixed with labelled with 4% paraformaldehyde and labeled with AlexaFluor488 phalloidin, rabbit anti alpha-catenin primary antibodies and Cy5 anti-rabbit secondary antibodies, mouse anti Phospho-MLC Ser18/Thr19 antibodies (Cell Signaling technologies) and mouse anti cy2 secondary antibodies. Images were acquired on a Zeiss LSM510 inverted microscope using a 63x 1.4 Numeric aperture oil immersion objective.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
keratinocyte
Cellular Component
actin filament
myosin light chain
alpha catenin
adherens junction
Biological Context
Biological Process
phosphorylation of myosin light chain
calcium stimulation
Attribution
Names
Ann Wheeler
OTHER
Queen Mary University London
Advanced Light Microscopy Facility
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35618
Archival Resource Key (ARK)
ark:/b7295/w9cil35618
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 740px 0.143µm
Y 1023px 0.143µm