Alternate header for print version

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35560*  Cite 

Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. Seen is a longitudinal section through a chromatid pair with kinetochore elements. This image recorded with a specimen tilt of 75 degrees is part of a grouped tilt series ranging from 20 degrees to 75 degrees. Pairs providing oblique stereo views of chromosome organization.

Technical Details

Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
Cell Line
Cellular Component
nuclear chromosome
Biological Context
Biological Process
Hans Ris
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Sample Preparation
formaldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
microtome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 4062px 1nm
Y 2561px 1nm