The Cell: An Image Library - Image CIL:35553

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CIL:35553^*

Description

Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. This image was recorded with a specimen tilt of 45 degrees. Grouped with it is one recorded at 55 degrees, providing an oblique stereo view of chromosome organization.

Technical Details

Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.

Biological Sources

NCBI Organism Classification: Cricetulus griseus
Cell Type: ovary
Cell Line: CHO
Cellular Component: nuclear chromosome

Biological Context

Biological Process: mitosis

Attribution

Name: Hans Ris

Grouping

This image is part of a group.

Imaging

Image Type: film
Imaging Mode: FBbi:00000622
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4144px	1.0nm
Y	2781px	1.0nm

*CIL – Cell Image Library accession number. Please use this to reference an image.