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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35461*  Cite 

Chinese Hamster Ovary (CHO) cells were metaphase-arrested with colcemid, exposed to hypotonic salt to swell the cells and chromosomes, fixed, embedded in plastic, and sections observed by high voltage electron microscopy (HVEM). The chromatid pair seen in the image was recorded at a specimen tilt angle of 70 degrees. Grouped with this image are tilts of 41, 50, and 60 degrees, providing oblique stereo views of the chromatids.

Technical Details

Metaphase arrested cells were swollen in 0.07 M KCl, fixed with 10% paraformaldehyde, post-fixed with OsO4, dehydrated, embedded in plastic, and 0.25 micron sections obtained. Sections were observed with the Wisconsin HVEM operated at 1 MeV, and micrographs taken at four tilt angles. See Ris, H (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
Cell Line
Cellular Component
nuclear chromosome
Biological Context
Biological Process
Hans Ris
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Spatial Axis Image Size Pixel Size
X 4218px 1nm
Y 2832px 1nm