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Description
Time-lapse microscopy of mouse oocytes expressing YFP-TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2) and injected with a doublestranded siRNA against TPX2, siTPX2-5h. Images were taken every 30 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner. Video S7. Found at: doi:10.1371/journal.pone.0003338.s007
Technical Details
Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
Biological Sources
- NCBI Organism Classification
- Mus musculus
- (house mouse)
- Cell Type
- oocyte
- Cellular Component
- microtubule
- microtubule organizing center part
- nuclear chromosome
Biological Context
- Biological Process
- meiotic spindle organization
- microtubule organizing center organization
- RNA interference
- Molecular Function
- GTPase activity
Attribution
- Names
- Stephane Brunet
- Julien Dumont
- Karen W. Lee
- Kazuhisha Kinoshita
- Pascale Hikal
- Oliver J. Gruss
- Bernard Maro
- Marie-Helene Verlhac
- Link
- http://www.plosone.org/article/info:do...
- Pubmed
- 18833336
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- DsRed
- Processing History
- unprocessed raw data
- Data Qualifiers
- raw, unprocessed data
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 150px | —— |
| Y | 150px | —— |
| Time | 1800 sec | 34 |
*CIL – Cell Image Library accession number. Please use this to reference an image.